Genetics:DNA Cloning
Wednesday, September 17th, 2008Genetics is study and science of heredity and genetic variation in living organisms. DNA is a protein that is encoded with particular inforamtion to pass on from generation to generation. Whne these are mixed together one gets the practice of DNA cloning. This is a process that works in effort to reproduce DNA fragments. There are multiple steps to take in this process: DNA recombination; transformation; and selective amplification. DNA recombination is the simple process of placing a DNA fragment into a vector, which is basically a host containing an anitbiotic-resistance gene. From there the recombinant vector is placed into an e. coli cell treated with CCl(2), thus they can then be proliferated throught selective amplification, or reproduced in a selective manner. The cells without the vectors in it die out. This allows certain DNA fragments to be reprocude in a bulk.
Cited Source: http://www.web-books.com/MoBio/Free/Ch9A.htm
